𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Prediction of aqueous phase pH for enzymatic synthesis of peptides in aqueous–organic biphasic systems

✍ Scribed by Ke-Ke Fan; Pingkai Ouyang; Xijun Wu; Zuhong Lu

Publisher
Wiley (John Wiley & Sons)
Year
2001
Tongue
English
Weight
113 KB
Volume
76
Category
Article
ISSN
0268-2575

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

An approach has been developed for predicting the change of the aqueous phase pH due to the partition of amino acid substrates in aqueous–organic biphasic systems. In the model reaction of papain‐catalyzed synthesis of N‐benzyloxycarbonyl‐glycine‐L‐phenylalanine methyl ester (ZGPM) in an aqueous–ethyl acetate system, the effects of the initial aqueous phase pH and phase volume ratio on the final aqueous phase pH, and the change of the final aqueous phase pH during the course of ZGPM synthesis, are investigated. The results suggest that the approach is useful for selecting optimum reaction conditions for peptide synthesis not only in the aqueous–organic biphasic systems with free or immobilized enzymes but also with an immobilized enzyme in an organic solvent.

© 2001 Society of Chemical Industry

📜 SIMILAR VOLUMES

Continuous enzymatic production of pepti
Continuous enzymatic production of peptide precursor in aqueous/organic biphasic medium
✍ Yoshihiko Murakami; Tatsunori Yoshida; Shigemitsu Hayashi; Akira Hirata 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 205 KB 👁 1 views

N-(benzyloxycarbonyl)-L-aspartic acid (Z-L-Asp) has generally been used as a carboxyl substrate for the enzymatic synthesis of a precursor of aspartame (synthetic sweetener); however, alternative inexpensive protection groups have been in demand for lowering the total cost of its industrial-scale pr