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Studies on a naturally occurring human antibody active against mouse landschutz ascites tumor cells: II. Identification of the antibody as igm and its partial purification

✍ Scribed by R. Laskov; E. Simon; D. Ram; J. Gross

Publisher: John Wiley and Sons
Year: 1968
Tongue: French
Weight: 583 KB
Volume: 3
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910030113

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✦ Synopsis

The cytotoxic antibody in normal human serum, active against mouse tumor cells, was identified as a 19s macroglobulin by means of Sephadex gel filtration, DEAEcellirlose chromatography and density-gradient centrifugation procedures.

A purified IgM fraction was isolated ji-om normal human serum. The cytotoxic activity of' this jiaction, as tested against mouse tumor cells, was enriched 20-80 times over that of the unfractionated serum. The purified IgM showed mainly one precipitin line on immunoelectrophoretic analysis. In the ultracentrifuge the 19s peak comprised about 85 2.

of' rhe totai protein.

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A quantitative cytolytic assay system was developed for the titration of the natural cytotoxin of human serum ( N H S j which is active against Landschutz ascites tumor cells. The assay system consists of NHS,, or N H S as a source of antibody and human umbilical cord serum as a source of complement