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Studies on a naturally occurring human antibody active against mouse landschutz ascites tumor cells: I. Cytotoxicity assay system and quantitation in normal sera

✍ Scribed by R. Laskov; E. Simon; J. Gross

Publisher: John Wiley and Sons
Year: 1968
Tongue: French
Weight: 700 KB
Volume: 3
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910030112

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✦ Synopsis

A quantitative cytolytic assay system was developed for the titration of the natural cytotoxin of human serum ( N H S j which is active against Landschutz ascites tumor cells. The assay system consists of NHS,, or N H S as a source of antibody and human umbilical cord serum as a source of complement.

' Mean f standard deviation

📜 SIMILAR VOLUMES

Studies on a naturally occurring human a

Studies on a naturally occurring human antibody active against mouse landschutz ascites tumor cells: II. Identification of the antibody as igm and its partial purification

✍ R. Laskov; E. Simon; D. Ram; J. Gross 📂 Article 📅 1968 🏛 John Wiley and Sons 🌐 French ⚖ 583 KB

## The cytotoxic antibody in normal human serum, active against mouse tumor cells, was identified as a 19s macroglobulin by means of Sephadex gel filtration, DEAEcellirlose chromatography and density-gradient centrifugation procedures. A purified IgM fraction was isolated ji-om normal human serum.

Studies on a naturally occurring human a

Studies on a naturally occurring human antibody active against mouse landschutz ascites tumor cells III. The nature of the antigenic site on the tumor cell

✍ R. Laskov; E. Simon; J. Gross 📂 Article 📅 1968 🏛 John Wiley and Sons 🌐 French ⚖ 829 KB

## Each value in the Table is the arithmetic mean of the values obtained in two similar experiments. The amount of antibody activity present in the unabsorbed serum was taken as 100:1,. The ratio between the specific activities (in LD,, unitsjmg) of the eluate and the unabsorbed serum.