The structure of the capsular polysaccharide from Klebsiellu K50 has been determined by using the techniques of methylation, periodate oxidation, partial hydrolysis, and p-elimination. N.m.r. spectroscopy (iH and 13C) was used to establish the nature of the anomeric linkages. The polysaccharide is
Studies of the primary structure of the capsular polysaccharide from Klebsiella serotype K15
β Scribed by Karunamay Nath; Ajit K. Chakraborty
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 619 KB
- Volume
- 161
- Category
- Article
- ISSN
- 0008-6215
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β¦ Synopsis
The capsular polysaccharide of Klebsiella serotype K15 has been investigated mainly by methylation analysis, characterisation of the oligosaccharides obtained by partial acid hydrolysis, periodate oxidation, enzymic degradation, and 1H- and 13C-n.m.r. spectroscopy, and shown to have the hexasaccharide repeating-unit 1. The glycan does not contain any pyruvic acetal or O-acetyl substituents. (formula; see text)
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The capsular polysaccharide from Klebsiella K44 has been investigated by the techniques of methylation, base-catalyzed elimination, Smith degradation, and partial hydrolysis. The last-named yielded an oligosaccharide corresponding to one repeating unit. The anomeric configurations of the sugar resid
The determination of the structure of the capsular polysaccharide from Klebsiella K67 involved the use of methylation, periodate oxidation, partial hydrolysis, and &elimination. The nature of the anomeric linkages was established by using 'H-and '"C-n.m.r. spectroscopy, and was further confirmed by
The structure of the capsular polysaccharide from Klebsiella type K43 has been investigated using sugar and methylation analysis, uranic acid degradation, and NMR spectroscopy on the native and the O-deacetylated polysaccharide. It is concluded that the polysaccharide is composed of pentasaccharide
The structure of the capsular polysaccharide elaborated by KlebsieUa type 7 has been investigated. NMR spectroscopy together with sugar and methylation analysis have been the main methods used. A uronic acid degradation was also employed. The polysaccharide consists of hexasaccharide repeating units