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Studies of the plasma membrane of normal and virus-transformed 3T3 mouse cells

✍ Scribed by Rose Sheinin; K. Onodera

Book ID
115721817
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
883 KB
Volume
274
Category
Article
ISSN
0005-2736

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Cell cycle dependent modulations of the
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## Abstract Agglutinability with Concanavalin was studied as function of cell cycle transition in normal and SV40 virus transformed 3T3 cells. In synchronized cultures of normal cells, agglutinbility was high during mitosis and disappeared rapidly. Agglutinability of transformed cells remained high

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3T3 and SV-40 transformed 3T3 mouse fibroblasts were cultured i n media with serum and antibiotics plus ammonia (NH3 z NH,') added as NHaC1. Both cell lines cultured without added ammonia showed normal morphology and multiplication even though ammonia in the medium at the end of the culture period r

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## Abstract The transport and phosphorylation of 2‐deoxy‐D‐glucose are separate and sequential events in both normal and virus‐transformed 3T3 cells. The apparent enhancement of 2‐dOG uptake by 3T3 cells accompanying virus transformation is not due to an effect on the transport process but to enhan