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Phosphorylation but not transport of sugars is enhanced in virus-transformed mouse 3T3 cells

✍ Scribed by C. Colby; A. H. Romano

Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 679 KB
Volume: 85
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040850103

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✦ Synopsis

Abstract

The transport and phosphorylation of 2‐deoxy‐D‐glucose are separate and sequential events in both normal and virus‐transformed 3T3 cells. The apparent enhancement of 2‐dOG uptake by 3T3 cells accompanying virus transformation is not due to an effect on the transport process but to enhanced phosphorylation by intracellular kinases. Phosphorylation of 3‐O‐methyl‐D‐glucose does not occur in these cells. Both the rate and extent of transport of this glucose analog is the same in normal cells, SV~40~ virus‐transformed cells and sarcoma virus‐transformed cells. The appropriateness of using 3‐O‐MeG for studies of the glucose transport system of animal cells is examined and discussed.

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The effect of serum on the transport and

The effect of serum on the transport and phosphorylation of 2-deoxyglucose by untransformed and transformed mouse 3T3 cells

✍ John A. Hassell; Clarence Colby; Antonio H. Romano 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 English ⚖ 683 KB

## Abstract Serum starvation of growing and nongrowing (density‐inhibited) mouse 3T3 cells resulted in decreased phosphorylation of 2‐deoxy‐D‐glucose. while the time course of transport of this sugar remained unchanged. Serum starvation of SV40 transformed 3T3 cells (SV101) and spontaneously transf