✦ LIBER ✦

Cell cycle dependent modulations of the surface membrane of normal and SV40 virus transformed 3T3 cells

✍ Scribed by L. A. Smets; L. De Ley

Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 479 KB
Volume: 84
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040840303

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✦ Synopsis

Abstract

Agglutinability with Concanavalin was studied as function of cell cycle transition in normal and SV40 virus transformed 3T3 cells. In synchronized cultures of normal cells, agglutinbility was high during mitosis and disappeared rapidly. Agglutinability of transformed cells remained high in G~1~ phase but diminished gradually upon entering S phase and reached minimum in G~1~ phase. Decreased agglutinability a the end of the cell cycle was also observed in synchronous SV3T3 cultures by a combined technique of haemadsorption and density gradient centrifugation. In normal 3T3 cells, similar variations in agglutin ability during interphase could not be observed.

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