๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Studies of non-covalent interactions of actinomycin D with single-stranded oligodeoxynucleotides by ion spray mass spectrometry and tandem mass spectrometry

โœ Scribed by Yin Liang Hsieh; Yu-Tsyr Li; Jack D. Henion; Bruce Ganem

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
435 KB
Volume
23
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

โœฆ Synopsis

Non-covalent binding of actinomycin D (AMD) to single-stranded DNA (ssDNA) was observed by ion spray mass spectrometry. Interactions between AMD and different sequences of non-self-complementary oligodeoxynucle+ tides were investigated as a function of p R Non-covalent AMDlssDNA complexes disappeared at low pH, and no ssDNA complexes were detected with rifampicin (RP). Moreover, the different binding affinities between AMD and =DNA with and without "GC" or "C-G" base sequences were also demonstrated using ion spray mass spectrometry. Additional support for the noncovalent nature of binding in AMD/ssDNA complexes was obtained from tandem mass spectrometry.

๐Ÿ“œ SIMILAR VOLUMES

Determination of non-covalent metal ion-
Determination of non-covalent metal ion-protein interactions using a microflow electrospray ionization mass spectrometry interface
โœ Kenneth L. Johnson; Timothy D. Veenstra; Andy J. Tomlinson; Rajiv Kumar; Stephen ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 126 KB ๐Ÿ‘ 2 views

Figure 3. Deconvoluted spectra of rat brain calbindin D 28K showing (A) the apoprotein and (B) the holoprotein showing four calcium bound.

Quantitative Determination of Orlistat(T
Quantitative Determination of Orlistat(Tetrahydrolipostatin, Ro 18-0647) in HumanPlasma by High-performance LiquidChromatography Coupled with Ion Spray Tandem Mass Spectrometry
โœ Bennett, Patrick K.; Li, Yu-Tsyir; Edom, Richard; Henion, Jack ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 355 KB

A rapid, sensitive and speciรc analytical method was developed and validated to quantify tetrahydrolipostatin (Orlistat, Ro 18-0647) in human plasma in order to provide pharmacokinetic data from clinical trials. This method employs a preliminary plasma protein precipitation step followed by a simple

Non-covalent complexes of nucleosides an
Non-covalent complexes of nucleosides and nucleobases with ฮฒ-cyclodextrin: a study by fast atom bombardment mass spectrometry and collision-induced dissociation
โœ Madhusudanan, K. P.; Katti, S. B.; Dwivedi, A. K. ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 255 KB ๐Ÿ‘ 1 views

Naturally occurring nucleosides and nucleobases form inclusion complexes with b-cyclodextrin. These host-guest complexes could be detected by fast atom bombardment mass spectrometry. The collision-induced dissociation spectra of the protonated complexes showed mainly ions related to the guest molecu

Multicomponent Non-covalent Associations
Multicomponent Non-covalent Associations of ฮฒ-Cyclodextrin (ฮฒ-CD)-Drug Inclusion Complexes with Diethanolamine (DEA).Detection and Characterization of Gaseous Protonated 1:1:1 ฮฒ-CD-Drug-DEA Adducts by Ionspray Ionization and Tandem Mass Spectrometry
โœ Selva, Antonio; Redenti, Enrico; Ventura, Paolo; Zanol, Margherita; Casetta, Bru ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 428 KB ๐Ÿ‘ 1 views

The multicomponent non-covalent associations of fbcyclodextrin (p-CD)-drug host-guest complexes with appropriate molecules are of current interest to the pharmaceutical industry, as they can increase dramatically the solubility in water of scarcely soluble guest drugs. The present study by ionspray