The conversion of a tol I11 Escherichia coli mutant, tolerant to colicins E2, E3, I a and K, into protoplast-like stable L-form makes i t sensitive to colicins E2, I a and K. I t s original sensitivity t o colicin El and tolerance to E3 are preserved in this celf form. Thus, rods of this mutant are
Studies of colicin action on wall-less stable L-forms of Escherichia coli II. Growth inhibition of complete and wall-less (L-form) cells of Escherichia coli by basic colicin types
✍ Scribed by Dr. J. Šmarda; Edith Schuhmann†
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 404 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
The inhibitive activity of colicins of 16 types (produced by 22 colicinogenic strains) on rods and protoplast-like stable L-formcellsof the strains Escherichia coli B, W1655 F' and W1655 Fwas compared. The results of 58 combinations tested fall into four groups (with three subgroups): 1) both cell forms are sensitive a) both cell forms are about equally sensitive b) rods are distinctly more sensitive than L-form cells c) L-form cells are distinctly more sensitive than rods 2) only rods are sensitive, L-form cells are not sensitive 3) only L-form cells are sensitive, rods are not sensitive 4) neither cell form is sensitive.
📜 SIMILAR VOLUMES
Colicin E3 has been shown t o be a n unique ribonuclease: it blocks the proteosynthesis in susceptible bacteria through the cleavage of a 3'-terininal fragment from the 16s ribosomal RNA in the 30s ribosomal subunits (SENIOR and HOLLAND 1971, BOWMAN et al. 1971 a). The role of individual surface str
## Studies of colicin action on wall-less stable L-forms of Escherichia coli I. Degree of attachment and of killing effect on rods and stable L-form cells J. SMARDA and EDITH SCHUHMANX? (Eingeyanyen urn 27. 10.1978) h'scherichia coli strains B and K12 W 1655 F+ are able t.0 bind more lethal units