The conversion of a tol I11 Escherichia coli mutant, tolerant to colicins E2, E3, I a and K, into protoplast-like stable L-form makes i t sensitive to colicins E2, I a and K. I t s original sensitivity t o colicin El and tolerance to E3 are preserved in this celf form. Thus, rods of this mutant are
An ambiguous effect of colicin E3 on wall-less stable L-form cells of Escherichia coli
✍ Scribed by MUDr. J. Šmarda; Edith Schuhmann
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 233 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Colicin E3 has been shown t o be a n unique ribonuclease: it blocks the proteosynthesis in susceptible bacteria through the cleavage of a 3'-terininal fragment from the 16s ribosomal RNA in the 30s ribosomal subunits (SENIOR and HOLLAND 1971, BOWMAN et al. 1971 a). The role of individual surface structures of bacterial cells, mediating this effect of colicin E3 -similarly as of other colicinsi . e . the localization and function of colicin receptors, has, however, not been understood so far.
According t o the commonly accepted view, colicin receptors like those of bacteriophages are situated in the cell wall. I n fact,, component's of the cell walls of susceptible bacteria are able t o adsorb large amounts of colicins (e.g.
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The inhibitive activity of colicins of 16 types (produced by 22 colicinogenic strains) on rods and protoplast-like stable L-formcellsof the strains Escherichia coli B, W1655 F' and W1655 Fwas compared. The results of 58 combinations tested fall into four groups (with three subgroups): 1) both cell f
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