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Structure de la mycosubtiline, antibiotique isolé de Bacillus subtilis

✍ Scribed by Françoise PEYPOUX; Georges MICHEL; Lucien DELCAMBE

Book ID
115115268
Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
628 KB
Volume
63
Category
Article
ISSN
1432-1327

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The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. su