Analyse génétique de sacB, gène de structure d'une enzyme secrétée, la lévane-saccharase de Bacillus subtilis Marburg

✍ Scribed by Steinmetz, Michel ;Le Coq, Dominique ;Djemia, Hajer Ben ;Gay, Philippe

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 936 KB
• Volume: 191
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00330901

✦ Synopsis

The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. subtilis chromosome. Using a collection of plasmids bearing various deletions extending in sacB we developed a technique of deletion mapping based on plasmid integration in the chromosome of B. subtilis. A generalization of this technique is discussed.