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Iodation de la levane-sucrase de bacillus subtilis

✍ Scribed by Georges Rapoport; Jean-Régis Chambert; Raymond Dedonder

Book ID
115731447
Publisher
Elsevier Science
Year
1967
Weight
541 KB
Volume
132
Category
Article
ISSN
0005-2744

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The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. su