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Structure and function of the repressor of bacteriophage lambda

✍ Scribed by Das, Tapas ;Mandal, N. C.

Publisher
Springer
Year
1986
Tongue
English
Weight
302 KB
Volume
204
Category
Article
ISSN
0026-8925

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✦ Synopsis

The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively, the level of repressor in a monolysogen of lambda cIha. Results of the study of certain properties of the bacteria carrying these plasmids suggest that the ha repressor also has a higher affinity for the virulent mutant operators as well as the prm promoter of lambda.

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