On maturation of the bacteriophage lambda chromosome
β Scribed by Feiss, Michael ;Margulies, Thomas
- Publisher
- Springer
- Year
- 1973
- Tongue
- English
- Weight
- 536 KB
- Volume
- 127
- Category
- Article
- ISSN
- 0026-8925
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## Abstract When phage DNA is added to an extract of an induced lambda lysogen, complete phage particles are made that contain the added DNA. The DNA substrate for packaging is a covalently joined polymer of several phage units. Unjoined units must first be joined by DNA ligase in the extract. Ther
The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively,
By mutagenizing a 2cIts (2ci857) lysogen, a 2 mutant has been isolated with a wild-type phenotype. This mutant phage lysogenizes with low efficiency and produces a low burst. Though the initial rates of repressor synthesis in Escherichia coli after infection with wild-type and mutant 2 are the same,