Studies were made on two guanine-requiring strains of Escherichia coli isolated independently as a result of insertion of prophage gamma into one of the structural genes of the guanine operon. These mutants do not exhibit any detectable guaB function but express the guaA function constitutively at a
Mechanism of transcription in the N operon of bacteriophage lambda
✍ Scribed by Portier, Marie-Madeleine ;Marcaud, Lise ;Cohen, Arlette ;Gros, François
- Publisher
- Springer
- Year
- 1972
- Tongue
- English
- Weight
- 576 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The maize chloroplast gene coding for the large subunit of ribulose bisphosphate carboxylase (3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39) has been placed under the transcriptional control of the bacteriophage lambda promoter PL, by fusion with the lambda N operon located on a mult
A bacteriophage lambda cloning vector carrying the trp/lacW205 substitution is described. The vector facilitates the fusion in vitro of genetic control signals to the lacZ structural gene of Escherichia coli. This system was used to define transcriptional termination sites in the lambda b2 region. T
The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively,
By mutagenizing a 2cIts (2ci857) lysogen, a 2 mutant has been isolated with a wild-type phenotype. This mutant phage lysogenizes with low efficiency and produces a low burst. Though the initial rates of repressor synthesis in Escherichia coli after infection with wild-type and mutant 2 are the same,