The 0-polysaccharide from k%rio cholerae 0 : 5 has been investigated, using NMR spectroscopy as the main method. Fast atom bombardment mass spectrometry (FABMS) studies of fragments obtained on treatment with anhydrous hydrogen fluoride or methanolic hydrogen chloride gave further structural informa
Structural studies of the O-antigen polysaccharide of Salmonella thompson, serogroup C1 (6,7)
✍ Scribed by Bengt Lindberg; Karin Leontein; Ulf Lindquist; Stefan B. Svenson; Göran Wrangsell; Anne Dell; Mark Rogers
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 585 KB
- Volume
- 174
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The structure of the O-antigen polysaccharide of Salmonella thompson, serogroup C1 (6,7) has been investigated mainly by methylation analysis, n.m.r. spectroscopy, specific degradations by a phage-associated enzyme, N-deacetylation-deamination, and f.a.b.-m.s. It is concluded that the structure involves the following repeating unit. (formula; see text) There are two populations of chains, with and without alpha-D-glucopyranosyl groups, 3-linked to an alpha-D-Manp residue, and only the latter type is hydrolysed by the phage enzyme. The alpha linkage of the third Manp residue is cleaved by the O14 phage enzyme. The structure, with or without the alpha-D-glucopyranosyl group, represents the biological repeating-unit.
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