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Structural studies of the O-antigen polysaccharide of Salmonella thompson, serogroup C1 (6,7)

✍ Scribed by Bengt Lindberg; Karin Leontein; Ulf Lindquist; Stefan B. Svenson; Göran Wrangsell; Anne Dell; Mark Rogers


Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
585 KB
Volume
174
Category
Article
ISSN
0008-6215

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✦ Synopsis


The structure of the O-antigen polysaccharide of Salmonella thompson, serogroup C1 (6,7) has been investigated mainly by methylation analysis, n.m.r. spectroscopy, specific degradations by a phage-associated enzyme, N-deacetylation-deamination, and f.a.b.-m.s. It is concluded that the structure involves the following repeating unit. (formula; see text) There are two populations of chains, with and without alpha-D-glucopyranosyl groups, 3-linked to an alpha-D-Manp residue, and only the latter type is hydrolysed by the phage enzyme. The alpha linkage of the third Manp residue is cleaved by the O14 phage enzyme. The structure, with or without the alpha-D-glucopyranosyl group, represents the biological repeating-unit.


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