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Structural determination of lipid a from gram negative bacteria using laser desorption mass spectrometry

✍ Scribed by Robert J. Cotter; Jeffrey Honovich; Nilofer Qureshi; Kuni Takayama

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 601 KB
Volume: 14
Category: Article
ISSN: 1076-5174
DOI: 10.1002/bms.1200141103

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✦ Synopsis

Laser desorption mass spectrometry has been employed for the structural determination of lipid A components derived from the lipopolysaccharides (LPS) of gram-negative bacteria. Mass spectra were obtained for methylated monophosphoryl lipid A from Neisseria gonorrhoeae and Rhodopseudomonas sphaeroides, for diphosphoryl lipid A from Escherichia coli and for the intact LPS from the Re Mutant of E . coli consisting of triphosphoryl lipid A and two KDO (2-keto-3-deoxyoctonate) units. Fragmentation of the phosphate (or pyrophosphate) on the reducing glucosamine is followed by fragmentation of acyl-linked fatty acids. Also observed are fragment ions which correspond to the distal portion of the molecule.

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