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Structural characterizations of lipids A by MS/MS of doubly charged ions on a hybrid linear ion trap/orbitrap mass spectrometer

✍ Scribed by Alba Silipo; Cristina De Castro; Rosa Lanzetta; Antonio Molinaro; Michelangelo Parrilli; Giorgio Vago; Luisa Sturiale; Angela Messina; Domenico Garozzo

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
163 KB
Volume
43
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Here, a new ‘one pot’ and fast approach is described, based on electrospray ionization (ESI) of negative ions by using a hybrid linear ion trap/orbitrap mass spectrometer (LTQ/orbitrap) for MS and MS/MS analysis. By this method the distribution of the primary and secondary acyl residues of the intact lipid A is inferred by analysis of the ESI spectra measured in positive and negative mode. The analysis of these data allows an unequivocal assignment of the fatty acid distribution. This methodology was successfully tested on two different lipid A with known structures, deriving from the Agrobacterium tumefaciens and Escherichia coli lipopolysaccharides (LPS). Copyright © 2007 John Wiley & Sons, Ltd.

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