Structural and functional analysis of the origin of replication of mitochondrial DNA fromParamecium aurelia

✍ Scribed by A. E. Pritchard; D. J. Cummings

• Publisher: Springer-Verlag
• Year: 1984
• Tongue: English
• Weight: 517 KB
• Volume: 8
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00410432

✦ Synopsis

Initiation of replication of the linear mitochondrial DNA from Paramecium occurs at a unique cross-linked terminus of the monomer molecule. Dimer length molecules, containing head-to-head monomers, are replicative intermediates. Previous studies have been with cloned dimer initiation region fragments but here we have isolated and sequenced isomeric forms of restriction fragments located at the initiation end of the monomer. The sequence isomers are inverted complements of each other in the region identified as the singlestranded DNA terminal loop. The unusual electrophoretic behaviour of these terminal restriction fragments supports the sequence data result that the loop is single stranded. These structural features are discussed in regard to mechanisms for the processing of dimer to monomer molecules.