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Structural analysis of the rat T-cell receptorTcra V4gene family

✍ Scribed by Martin Stangel; Gerhard Giegerich; Nora E. Torres-Nagel; Thomas Hünig; Hans-Peter Hartnug

Book ID
104653733
Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
613 KB
Volume
41
Category
Article
ISSN
0093-7711

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✦ Synopsis

The rat Tcra V gene locus is only poorly characterized, although rats are widely used in a variety of T-cell-mediated experimental animal models. Recently, we described the first monoclonal antibody, G99, directed against a rat Tcra V4 segment. We examined cDNA transcripts of G99-positively sorted T cells and show that the monoclonal antibody G99 most likely recognizes at least two members of the Tcra V4 family. Moreover, we analyzed the genomic repertoire of this VA family and report 15 novel Tcra V4 DNA sequences. Based on sequence and Southern blot analysis, the Tcra V4 family could be divided into four subgroups, which were also detected in mice. These findings corroborate previous findings of a similar genetic organization of the Tcra V loci in both species.

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Structural analysis of the mouse T-cell receptorTcra V2subfamily
✍ Kai Wang; Chia-Lam Kou; Kuang-Chuan Cheng; Mi-Kyung Lee; Bryan Paeper; Ben F. Ko 📂 Article 📅 1994 🏛 Springer-Verlag 🌐 English ⚖ 711 KB

Cosmid clones containing T-cell receptor Tcra V2 subfamily gene segments have been isolated from a BALB/ c cosmid library and subjected to DNA sequence analysis. The V gene segments in the Tcra V2 subfamily differ from each other by 3%-7% at the nucleotide level and 5% -16% at the amino acid level.