✦ LIBER ✦

Structural analysis of the mouse c-HA-ras gene promoter

✍ Scribed by Mark Plumb; Jean-Baptiste Telliez; Frances Fee; Pierre Daubersies; Bernard Bailleul; Allan Balmain

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 830 KB
Volume: 4
Category: Article
ISSN: 0899-1987
DOI: 10.1002/mc.2940040205

No coin nor oath required. For personal study only.

✦ Synopsis

Previous studies have demonstrated that the mouse c-Harvey ras proto-oncogene (c-Ha-ras) promoter sequences are GC rich and contain several potential transcription factor SP1 binding sites. We investigated the endonuclease hypersensitivity of this region in nuclei in vitro and whole mouse tissues in vivo and identified a very strong, ubiquitous hypersensitive site covering the proximal promoter sequences. Footprint protection studies using nuclear extracts from various cell types including fibroblasts, erythroid cells, and both normal and transformed epithelial cells revealed a consistent protein-binding pattern. Five protein binding sites were observed, four of which correlated with potential SP1 binding sites. Competition experiments using an oligonucleotide corresponding to a consensus SP1 binding site confirmed that these sequences were indeed bound by the SP1 (or SP1-like) trans-acting factor. In addition, no differences were observed between the footprint patterns obtained using extracts from cells of different lineages or between normal and transformed epithelial cells carrying activated ras genes. The controlling elements responsible for differential c-Ha-ras transcription between cell types or at different stages of carcinogenesis therefore probably lie in other regions of the gene.

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