Structural Analysis of N-acetylglucosamine-6-phosphate Deacetylase Apoenzyme from Escherichia coli
✍ Scribed by Frederico M. Ferreira; Guillermo Mendoza-Hernandez; Maria Castañeda-Bueno; Ricardo Aparicio; Hannes Fischer; Mario L. Calcagno; Glaucius Oliva
- Book ID
- 116662951
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 879 KB
- Volume
- 359
- Category
- Article
- ISSN
- 0022-2836
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A single-vial assay has been developed for N-acetylglucosamine-6-phosphate deacetylase. in which ['HIacetate released from 'H-acetyl-labeled substrate is measured in a biphasic liquid scintillation counting system after acidification of the reaction mixture. The deacetylase was partially purified fr
## Abstract The __N__‐acetylglucosamine‐1‐phosphate uridyltransferase (GlmU) is a key bifunctional enzyme in the biosynthesis of UDP‐GlcNAc, a precursor in the synthesis of cell wall peptidoglycan. Crystal structures of the enzyme from different bacterial strains showed that the polypeptide forms a