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Purification and characterization of N-Acetylglucosamine 6-phosphate deacetylase from Thermus caldophilus

✍ Scribed by Hyun-Jae Shin; Mikyung Kim; Dae-Sil Lee

Book ID
114398635
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
413 KB
Volume
88
Category
Article
ISSN
1389-1723

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A single-vial assay has been developed for N-acetylglucosamine-6-phosphate deacetylase. in which ['HIacetate released from 'H-acetyl-labeled substrate is measured in a biphasic liquid scintillation counting system after acidification of the reaction mixture. The deacetylase was partially purified fr