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N-Acetylglucosamine-6-phosphate Deacetylase fromEscherichia coli:Purification and Molecular and Kinetic Characterization

✍ Scribed by José M. Souza; Jacqueline A. Plumbridge; Mario L. Calcagno

Book ID
115566931
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
256 KB
Volume
340
Category
Article
ISSN
0003-9861

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✍ Patrick Campbell; Torvard C. Laurent; Lennart Rodén 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 622 KB

A single-vial assay has been developed for N-acetylglucosamine-6-phosphate deacetylase. in which ['HIacetate released from 'H-acetyl-labeled substrate is measured in a biphasic liquid scintillation counting system after acidification of the reaction mixture. The deacetylase was partially purified fr