Strategies for the cryopreservation of microencapsulated cells
โ Scribed by Boon Chin Heng; Hanry Yu; Soon Chye Ng
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 22 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Cryopreservation of human hepatocytes is important for their use in hepatocyte transplantation. On thawing, cryopreserved hepatocytes often have reduced viability and metabolic function in comparison with fresh cells. The aim of this study was to modify the different steps in the standard cryopreser
We evaluated the AS104 cell separator (Fresenius AG, Bad Homburg, Germany) for ex vivo processing of bone marrow (BM) grafts of 43 patients suffering from germ cell cancer (GCC, n = 22), acute lymphocytic leukemia (ALL, n = 13) and malignant lymphoma (ML, n = 8). Recoveries of total nucleated cells
Bone marrow collection was undertaken from human organ donors (Group I ; n = 7) to develop a closed-system single-step technique for stem and progenitor cell enrichment, using the Cobc 2997 continuous-flow blood-cell separator. The effects of programmed freezing, storage in liquid nitrogen. and thaw
## Abstract During the past decade, stem cell transplant has emerged as a novel therapeutic alternative for several diseases. If therapies are to be implemented in clinical settings, efficient scaleโup of stem cells isolation methodologies would be crucial. A brief, processโoriented overview of the
## ABSTRACT Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25ยฐC and 3 hours recovery at 37ยฐC) improved hamster cell survival to freezeโthaw damage (p < 0.01) but decreased th