Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells.

✍ Scribed by B. Kaeffer; I.Garcia Uriel; E. Bottreau

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 337 KB
• Volume: 18
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1994.1029

✦ Synopsis

ABSTRACT

Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25°C and 3 hours recovery at 37°C) improved hamster cell survival to freeze‐thaw damage (p < 0.01) but decreased the survival of 2 immortal pig ileal cell lines even though epithelioid IPI‐2I cells were more tolerant to hypothermia than IPI‐I fibroblasts. Epithelioid cells survived 3 days at 25°C with unaltered expression of cytokeratin‐18 whereas colonies of fibroblasts did not survive more than a day at 25°C (p < 0.001). These results suggest that hypothermia‐tolerance of pig ileal cell lines might differ according to cell lineage calling for further experiments on small intestinal primary cell culture.