Storage and preservation of whole blood samples for use in detection of human immunodeficiency virus type-1 by the polymerase chain reaction
β Scribed by Steve Kaye; Clive Loveday; Richard S. Tedder
- Book ID
- 119115050
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 687 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0166-0934
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π SIMILAR VOLUMES
An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR
## Abstract A highly sensitive twoβstep polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIVβ1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna