𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of human immunodeficiency virus type 1 genomic RNA in plasma samples by reverse-transcription polymerase chain reaction

✍ Scribed by Patrizia Bagnarelli; Stefano Menzo; Aldo Manzin; Mauro Giacca; Pietro Emanuele Varaldo; Massimo Clementi

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
762 KB
Volume
34
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR) from seropositive subjects. The assay is faster and cheaper than detection of specific HIV-1 transcripts from peripheral blood mononuclear cells by RT-PCR. The data suggest that HIV-1 viremia is detectable by plasma RT-PCR in a large proportion of seropositive individuals.

📜 SIMILAR VOLUMES

Detection of GB Virus-C/Hepatitis G Viru
Detection of GB Virus-C/Hepatitis G Virus RNA in Serum by Reverse Transcription Polymerase Chain Reaction
✍ Bhardwaj, Bhavna; Qian, KePing; Detmer, Jill; Mizokami, Masashi; Kolberg, Janice 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 202 KB 👁 2 views

Three PCR methods based on the GB virus-C/ hepatitis G virus (GBV-C/HGV) 5ЈUTR and NS3 genomic region were used for the detection of GBV-C/HGV RNA in serum of 62 patients with chronic hepatitis C virus (HCV) infection. Ten of 62 (16%) patients were found to have GBV-C/ HGV RNA, which was confirmed b

Nested polymerase chain reaction for det
Nested polymerase chain reaction for detection of human immunodeficiency virus type 1 DNA in clinical specimens
✍ Maurizio Zazzi; Laura Romano; Agostina Brasini; Pier Egisto Valensin 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 345 KB

## Abstract A highly sensitive two‐step polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIV‐1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna

Highly sensitive detection of viral RNA
Highly sensitive detection of viral RNA genomes in blood specimens by an optimized reverse transcription-polymerase chain reaction
✍ Shigeki Murakami; Yoshiyuki Takahashi; Shigeru Yoshida; Isao Fuke; Kozo Ohmae; C 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 722 KB

## Abstract A protocol was developed for a highly sensitive detection of viral RNA in blood specimens by reverse transcription coupled with a nested poly‐merase chain reaction. Using Japanese encephalitis virus (JEV) as a model, the optimized reverse transcription‐polymerase chain reaction (ORTPCR)

Polymerase chain reaction-based detectio
Polymerase chain reaction-based detection of hepatitis D virus genome in patients infected with human immunodeficiency virus
✍ Dr. Paul Dény; Catherine Lecot; Vincent Jeantils; Lydia Ovaguimian; Alain Krivit 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 528 KB

The polymerase chain reaction (PCR) was used to detect hepatitis D (HD) viremia in patients infected with the human immunodeficiency virus (HIV). Nineteen (9%) of 206 such patients, unselected for liver disease or HBV infection, were found prospectively to be infected by HDV. Thirty-one anti-HIV-pos

Detection of hepatitis C virus genome in
Detection of hepatitis C virus genome in formalin-fixed paraffin-embedded liver tissue by in situ reverse transcription polymerase chain reaction
✍ George K. K. Lau; Jane W. S. Fang; P. C. Wu; Gary L. Davis; Johnson Y. N. Lau 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 470 KB

## Abstract Detection of hepatic hepatitis C virus RNA and antigens is difficult since their expression is very low. The technique of in situ reverse transcription polymerase chain reaction (IS‐RT‐PCR) was developed for the detection and localization of HCV RNA in formalin‐fixed paraffin‐embedded l