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Highly sensitive detection of viral RNA genomes in blood specimens by an optimized reverse transcription-polymerase chain reaction

✍ Scribed by Shigeki Murakami; Yoshiyuki Takahashi; Shigeru Yoshida; Isao Fuke; Kozo Ohmae; Chisato Mori; Mitsuo Takagi; Akihisa Takamizawa; Hiroto Okayama

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
722 KB
Volume
43
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

A protocol was developed for a highly sensitive detection of viral RNA in blood specimens by reverse transcription coupled with a nested poly‐merase chain reaction. Using Japanese encephalitis virus (JEV) as a model, the optimized reverse transcription‐polymerase chain reaction (ORTPCR) detects as few as 3–5 virions in 0.1 ml of whole blood specimens. The effectiveness of this assay system is confirmed by diagnosis of human hepatitis C viral (HCV) infection. © 1994 Wiley‐Liss, Inc.

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