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Detection of GB Virus-C/Hepatitis G Virus RNA in Serum by Reverse Transcription Polymerase Chain Reaction

✍ Scribed by Bhardwaj, Bhavna; Qian, KePing; Detmer, Jill; Mizokami, Masashi; Kolberg, Janice A.; Urdea, Mickey S.; Schlauder, George; Linnen, Jeffrey M.; Kim, Jungsuh P.; Davis, Gary L.; Lau, Johnson Y. N.

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
202 KB
Volume
52
Category
Article
ISSN
0146-6615

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✦ Synopsis

Three PCR methods based on the GB virus-C/ hepatitis G virus (GBV-C/HGV) 5ЈUTR and NS3 genomic region were used for the detection of GBV-C/HGV RNA in serum of 62 patients with chronic hepatitis C virus (HCV) infection. Ten of 62 (16%) patients were found to have GBV-C/ HGV RNA, which was confirmed by sequence analysis of the 5ЈUTR PCR amplicon. All methods appear to be specific, but methods based on the 5ЈUTR appear to be more sensitive.

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