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Detection of human immunodeficiency virus type 1 transcripts in peripheral blood lymphocytes by the polymerase chain reaction

✍ Scribed by Patrizia Bagnarelli; Stefano Menzo; Aldo Manzin; Pietro E. Varaldo; Maria Montroni; Mauro Giacca; Massimo Clementi


Book ID
119115090
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
478 KB
Volume
32
Category
Article
ISSN
0166-0934

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## Abstract A highly sensitive two‐step polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIV‐1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna