Detection of human immunodeficiency virus type 1 transcripts in peripheral blood lymphocytes by the polymerase chain reaction
β Scribed by Patrizia Bagnarelli; Stefano Menzo; Aldo Manzin; Pietro E. Varaldo; Maria Montroni; Mauro Giacca; Massimo Clementi
- Book ID
- 119115090
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 478 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0166-0934
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR
## Abstract A highly sensitive twoβstep polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIVβ1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna