An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR
✦ LIBER ✦
Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction
✍ Scribed by I. Loussert-Ajaka; F. Simon; I. Farfara; D. Descamps; G. Collin; F. Brun-Vézinet
- Book ID
- 116158438
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 586 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0923-2516
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## Abstract Sapovirus (SaV) is an agent of gastroenteritis for humans and swine, and is divided into five distinct genogroups (GI–GV) based on its capsid gene sequences. Typical methods of SaV detection include electron microscopy (EM), enzyme‐linked immunosorbent assay (ELISA), and reverse transcr