✦ LIBER ✦

Stimulation of endothelial cell proliferation by precursors of thymidylate

✍ Scribed by B. R. McAuslan; W. Reilly; G. N. Hannan

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 567 KB
Volume: 100
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041000109

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Bovine corneal and aortal endothelial cell cultures were established from primary explants and subcultured for at least 40 passages. With both cell, exogenous thymidine, folate or folinate markedly increased the proliferation of these cells and decreased their serum requirement in Medium 199.

Medium 199 supplemented with thymidine was particularly useful for cell survival at low densities; clones were readily produced when single cells were plated as low as 0.07 cells. cm^−2^. In contrast to the results of others, neither fibroblast growth factor nor epidermal growth factor were necessary for cell proliferation or survival at low densities.

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