On the nature of the irreversible inhibi
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Karlheinz Weber; JΓ‘nos RΓ©tey
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Article
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1996
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Elsevier Science
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English
β 484 KB
The irreversible inhibition of histidine ammonia lyase by L-cysteine and dioxygen has been reexamined. After denaturation and consecutive digestion of the inhibited enzyme by trypsin and endoproteinase Glu-C, the generated chromophoric system (lambda max = 340 nm) remained intact and was isolated in