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The Inactivation of Histidine Ammonia-Lyase by l -Cysteine and Oxygen: Modification of the Electrophilic Center

✍ Scribed by Galpin, Jason D.; Ellis, Brian E.; Tanner, Martin E.


Book ID
126453354
Publisher
American Chemical Society
Year
1999
Tongue
English
Weight
145 KB
Volume
121
Category
Article
ISSN
0002-7863

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The irreversible inhibition of histidine ammonia lyase by L-cysteine and dioxygen has been reexamined. After denaturation and consecutive digestion of the inhibited enzyme by trypsin and endoproteinase Glu-C, the generated chromophoric system (lambda max = 340 nm) remained intact and was isolated in