✦ LIBER ✦

Stereospecific assignments of glycine in proteins by stereospecific deuteration and15N labeling

✍ Scribed by Robert W. Curley; Michael J. Panigot; Andrew P. Hansen; Stephen W. Fesik

Publisher: Springer Netherlands
Year: 1994
Tongue: English
Weight: 374 KB
Volume: 4
Category: Article
ISSN: 0925-2738
DOI: 10.1007/bf00179344

No coin nor oath required. For personal study only.

✦ Synopsis

A method is described for stereospecifically assigning the a-protons of glycine residues in proteins. The approach involves the stereospecific deuteration and lSN labeling of glycine and subsequent selective incorporation of this residue into the protein. The stereospecific assignments of the glycine c~-protons are obtained from a comparison of a 3D 15N-resolved TOCSY spectrum of the uniformly 15N-labeled protein with a 2D/3D 15N-edited TOCSY spectrum of the protein, containing the stereospecifically deuterated and ~SN-labeled glycine. The approach is demonstrated by stereospecifically assigning the glycine c~-protons of the FK506 binding protein when bound to the immunosuppressant ascomycin.

📜 SIMILAR VOLUMES

Determination of Stereospecific Assignme

Determination of Stereospecific Assignments, Torsion-Angle Constraints, and Rotamer Populations in Proteins Using the Program Anglesearch

✍ V.I. Polshakov; T.A. Frenkiel; B. Birdsall; A. Soteriou; J. Feeney 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 917 KB

Determination of the nitrogen configurat

Determination of the nitrogen configuration in ephedrine N-boranes and a study of their assisted stereospecific deuteration of NH

✍ Hugo Tlahuext; Fernando Santiesteban; Efren García-Báez; Rosalinda Contreras 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 529 KB

An (H)C(CO)NH-TOCSY pulse scheme for seq

An (H)C(CO)NH-TOCSY pulse scheme for sequential assignment of protonated methyl groups in otherwise deuterated15N,13C-labeled proteins

✍ Kevin H. Gardner; Robert Konrat; Michael K. Rosen; Lewis E. Kay 📂 Article 📅 1996 🏛 Springer Netherlands 🌐 English ⚖ 543 KB

Identification of sources of hydrogen at

Identification of sources of hydrogen atoms in fatty acids synthesized using deuterated water and stereospecifically deuterium labelled NADPH by gas chromatographic mass spectrometric analysis

✍ Yousuke Seyama; Akihiko Kawaguchi; Takeshi Kasama; Keizo Sasaki; Kazumi Arai; Sh 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 498 KB 👁 1 views

Sequence-Specific Assignment of Aromatic

Sequence-Specific Assignment of Aromatic Resonances of Uniformly 13C,15N-Labeled Proteins by Using 13C- and 15N-Edited NOESY Spectra

✍ Zhi Lin; Yingqi Xu; Shuai Yang; Daiwen Yang 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 281 KB 👁 1 views

Sequence-Specific Assignment of Aromatic

Sequence-Specific Assignment of Aromatic Resonances of Uniformly 13C,15N-Labeled Proteins by Using 13C- and 15N-Edited NOESY Spectra

✍ Zhi Lin; Yingqi Xu; Shuai Yang; Daiwen Yang 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 282 KB 👁 1 views

The precision of a protein structure determined by NMR spectroscopy is strongly influenced by the number of longrange NOE interactions per residue. Aromatic and methylcontaining residues are frequently located in the hydrophobic interior of proteins and yield long-range NOE interactions. Methyl grou