A JCH-Modulated 2D (HACACO)NH Pulse Sche
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T.Kevin Hitchens; Scott A. McCallum; Gordon S. Rule
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Article
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1999
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Elsevier Science
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English
β 73 KB
A triple-resonance pulse sequence is presented for the quantitative measurement of 1 H β£ -13 C β£ single-bond couplings in 15 N, 13 C uniformly labeled proteins. This 1 J CH -modulated (HACACO)NH experiment yields 1 H N -15 N-correlated 2D spectra in which the amplitude of each peak is modulated by t