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An (H)C(CO)NH-TOCSY pulse scheme for sequential assignment of protonated methyl groups in otherwise deuterated15N,13C-labeled proteins

✍ Scribed by Kevin H. Gardner; Robert Konrat; Michael K. Rosen; Lewis E. Kay


Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
543 KB
Volume
8
Category
Article
ISSN
0925-2738

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A JCH-Modulated 2D (HACACO)NH Pulse Sche
✍ T.Kevin Hitchens; Scott A. McCallum; Gordon S. Rule πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 73 KB

A triple-resonance pulse sequence is presented for the quantitative measurement of 1 H ␣ -13 C ␣ single-bond couplings in 15 N, 13 C uniformly labeled proteins. This 1 J CH -modulated (HACACO)NH experiment yields 1 H N -15 N-correlated 2D spectra in which the amplitude of each peak is modulated by t