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Stereoselective synthesis of stable isotope labeled L-α-amino acids: The enzymatic preparation of 13C-labeled L-glutamic acids

✍ Scribed by Warren J. Goux; Linda Rench; Denise S. Weber

Publisher
John Wiley and Sons
Year
1993
Tongue
French
Weight
617 KB
Volume
33
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

We have developed methods for the preparation of L‐glutamic acid, isotopically labeled with ^13^C, using commercially available purified enzymes. The procedure is sufficiently versatile that L‐glutamic acid may be labeled at any carbons or any combination of carbons using ^13^C‐labeled pyruvate, acetate and bicarbonate as isotopically labeled precursors. Using the strategy outlined, we have demonstrated the methodology using as an example the preparation of millimolar quantities of L‐[1‐^13^C], L‐[4‐^13^C], L‐[5‐^13^C] and L‐[1,3,4‐^13^C~3~]glutamic acid in a one‐pot incubation. We also outline methods for the preparation of ^13^C‐labeled L‐malate and citrate.

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