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Stepwise elution chromatography as a method for both purification and concentration of proteins

✍ Scribed by Shuichi Yamamoto; Masaki Nomura; Yuji Sano

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
429 KB
Volume
47
Category
Article
ISSN
0009-2509

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✦ Synopsis

This paper examines how stepwise elution (SE) chromatography can work as a method for the purification and the concentration of proteins at the same time. The concentration factors of proteins by SE with ion exchange chromatography (IEC) or hydrophobic interaction chromatography (HIC) columns were measured as a function of the feed volume and the fed concentration. The experimental results are in good agreement with the calculated ones on the basis of the adsorption isotherm data. The recovery of aamylase from the fermentation broth by SE-HIC was successfully carried out on the basis of the data obtained from the linear gradient elution experiments. The concentration factor obtained was 30-fold while the purification factor w& 23-fold.

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