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Purification of peanut proteins for further use in affinity chromatography and as immunogens

✍ Scribed by Sabine Baumgartner; Claudia Hemetsberger; Elisabeth Drs; Harald Pichler; Rudolf Krska

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
179 KB
Volume
26
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Chickens were immunised with peanut protein extracts. Because of the high cross reactivity, the lgY antibodies were purified by means of an affinity column. An ion chromatographically purified peanut protein fraction was therefore coupled to the affinity support material and this column was used for further lgY purification.

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