๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Purification of proteins and peptides for sequence analysis using microcolumn liquid chromatography

โœ Scribed by Robert L. Moritz; Richard J. Simpson

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
433 KB
Volume
4
Category
Article
ISSN
1040-7685

No coin nor oath required. For personal study only.

โœฆ Synopsis

Abstract

Reversed phase microcolumn (0.32 mm i.d.) liquid chromatography was used to purify lowโ€picomole amounts of proteins and peptides suitable for structural analysis. Using this approach, rapid trace enrichment (concentration) of low nanogram levels of proteins from volumes as high as 500 ฮผL down to 1โ€“2 ฮผL was demonstrated. The total system recovery (including manual collection and reinjection) for 50 ng of lysozyme was >95%; the overall recovery after five injections was 90%. Using the same packing, Brownlee RPโ€300, the resolution of a standard mixture of proteins was comparable for columns varying in length from 250 mm to 10mm. Identification by amino acid sequence analysis of selected peptides recovered from a subโ€10 pmol Staphylococcus aureus V8 protease digest of recombinant murine interleukinโ€6 (ILโ€6) was achieved.

๐Ÿ“œ SIMILAR VOLUMES

Rapid separation of proteins and peptide
Rapid separation of proteins and peptides by reversed-phase microcolumn liquid chromatography
โœ Norikazu Nagae; Hiroko Itoh; Noriyuki Nimura; Toshio Kinoshita; Toyohide Takeuch ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 357 KB ๐Ÿ‘ 1 views

Proteins and peptides were separated in the reversed-phase mode on microcolumns packed with nonporous octadecyl-group bonded silica gel with an average particle diameter of 4.5 or 20 pm. Separation columns were prepared from glass-lined stainless steel tubing of 39 or 56-mm x 0.5-mm i.d. An artifici

Weak cation-exchange monolithic column f
Weak cation-exchange monolithic column for capillary liquid chromatography of peptides and proteins
โœ Xin Chen; H. Dennis Tolley; Milton L. Lee ๐Ÿ“‚ Article ๐Ÿ“… 2011 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 432 KB

## Abstract A stable poly(2โ€carboxyethyl acrylateโ€__co__โ€poly(ethylene glycol) diacrylate) monolith was synthesized inside a 75โ€ฮผm id capillary by direct __in situ__ photoโ€initiated polymerization in a binary porogenic solvent consisting of methanol and ethyl ether. The resulting monolith was evalu

Purification of peanut proteins for furt
Purification of peanut proteins for further use in affinity chromatography and as immunogens
โœ Sabine Baumgartner; Claudia Hemetsberger; Elisabeth Drs; Harald Pichler; Rudolf ๐Ÿ“‚ Article ๐Ÿ“… 2003 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 179 KB

## Abstract Chickens were immunised with peanut protein extracts. Because of the high cross reactivity, the lgY antibodies were purified by means of an affinity column. An ion chromatographically purified peanut protein fraction was therefore coupled to the affinity support material and this column

Supercritical fluid extraction combined
Supercritical fluid extraction combined with microcolumn liquid chromatography for the analysis of polymer additives
โœ Y. Hirata; Y. Okamoto ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 319 KB

Polymer additives were extracted with supercritical carbon dioxide and collected in a microtrap. The collected samples were separated by microcolumn liquid chromatography. Polymer samples down to the sub-milligram level were handled without loss of extract. Information about the distribution of addi