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Rapid separation of proteins and peptides by reversed-phase microcolumn liquid chromatography

✍ Scribed by Norikazu Nagae; Hiroko Itoh; Noriyuki Nimura; Toshio Kinoshita; Toyohide Takeuchi

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
357 KB
Volume
3
Category
Article
ISSN
1040-7685

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✦ Synopsis

Proteins and peptides were separated in the reversed-phase mode on microcolumns packed with nonporous octadecyl-group bonded silica gel with an average particle diameter of 4.5 or 20 pm. Separation columns were prepared from glass-lined stainless steel tubing of 39 or 56-mm x 0.5-mm i.d. An artificial mixture of several proteins could be separated within 1 min by split-flow gradient elution. The system was applied to the separation of a triptic digest of mouse amyloid A. On-column sample enrichment allowed direct injection of 2-5 pL of sample solution, leading to improved detectability.

The limits of detection were < 1 pg mL-'.

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