𝔖 Bobbio Scriptorium
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Affinity chromatography as a rapid and convenient method for purification of fungal laccases

✍ Scribed by J. Rogalski; M. Wojtas-Wasilewska; R. Apalovič; A. Leonowicz

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
516 KB
Volume
37
Category
Article
ISSN
0006-3592

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✦ Synopsis

A rapid and convenient method for graduation, isolation, and purification of laccase from Trametes versicolor and Fomes fomentarius culture fluids was developed. For purification affinity chromatography on syringyl-and vanillylcontrolled porosity glass (CPG) columns was applied. The purified laccase of F. fomentarius was immobilized on porous glass. Some properties of the immobilized enzyme in comparison to the free one are discussed.

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