The distribution of nucleolar organizer regions (NORs) was studied in Papanicolaou preparations of cervical smears in order to distinguish benign from preneoplastic lesions. Destained smears (six defined as normal, six as inflammatory with squamous metaplasia, six as CIN I, six as CIN II, and five a
Staining of the nucleolar organizer regions: relevance in hematology
โ Scribed by S. Baatout
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 458 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0268-960X
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โฆ Synopsis
There is growing interest in staining for the nucleolar organizer regions to detect nucleolar organizer region-associated proteins. In some cases, this technique facilitates the study of hematological disorders and allows us to distinguish between certain pathologies. Furthermore, it can provide information about cell proliferation, activity and malignancy. This paper attempts to give the recent advances in the use of the staining of nucleolar organizer regions and its clinical relevance in hematology.
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A reliable technique for staining human chromosomal nucleolar organizers (NOR's) with silver solutions is described. The NOR's can be selectively stained dark brown by silver solutions leaving the chromosome arms unstained and available for counterstaining with orcein or Giemsa dyes. Unequivocal ide
Background. Nucleolar organizer regions (NORs) are loops of ribosomal DNA that occur in nucleoli and that transcribe to ribosomal RNA. NORs have been identified by means of the Ag-NOR technique in routinely processed tissues, and were found to be of discriminative value between some types of benign
Background. Nucleolar organizer regions (AgNORs) are associated with proliferative activity and ploidy in many tumors. The endocervical growth pattern of cervical adenocarcinoma renders tumor volume assessment more difficult, necessitating additional prognostic indicators. Methods. Thirty-five case