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An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes

✍ Scribed by S. E. Bloom; C. Goodpasture

Publisher: Springer
Year: 1976
Tongue: English
Weight: 871 KB
Volume: 34
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00278889

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✦ Synopsis

A reliable technique for staining human chromosomal nucleolar organizers (NOR's) with silver solutions is described. The NOR's can be selectively stained dark brown by silver solutions leaving the chromosome arms unstained and available for counterstaining with orcein or Giemsa dyes. Unequivocal identification of chromosome pairs bearing NOR's can be achieved using fluorescent banding techniques followed by silver staining. The silver staining procedure for NOR's was simplified and standardized through control of the chemical and physical conditions during silver impregnation and developing.

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