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Silver staining method for nucleolar organizer regions in cervical smears

✍ Scribed by Edenilson Eduardo Calore; Neuza Kasumi Shirata; Lai Wun Song Shih; Maria José Cavaliere; Marília de Siqueira

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
118 KB
Volume
16
Category
Article
ISSN
8755-1039

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✦ Synopsis

The distribution of nucleolar organizer regions (NORs) was studied in Papanicolaou preparations of cervical smears in order to distinguish benign from preneoplastic lesions. Destained smears (six defined as normal, six as inflammatory with squamous metaplasia, six as CIN I, six as CIN II, and five as CIN III) were submitted to the Ag-NOR method after staining with Orange G and EA36. Ag-NOR count was performed in previously outlined fields on the smears. Statistically significant differences (P , .05) were found between the normal smears, inflammatory smears with squamous metaplasia, and each grade of CIN. We conclude that the Ag-NOR technique could be useful to evaluate cervical smears of doubtful interpretation, using previous demarcation of the abnormal fields/cells.

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