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Staining of argininosuccinate lyase activity in polyacrylamide gel

✍ Scribed by Hwei-Jen Lee; Shyh-Horng Chiou; Gu-Gang Chang


Book ID
116098296
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
810 KB
Volume
24
Category
Article
ISSN
0165-022X

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Activity stain for pyruvate decarboxylas
✍ Hartmut Zehender; Dorothea Trescher; Johannes Ullrich πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 453 KB

A method for the localization of pyruvate decarboxylase bands in polyacrylamide gels after electrophoresis using 1,2-dianilinoethane in dilute acetic acid as reagent for acetaldehyde formed from pyruvate by the holoenzyme is described. A white condensation product forms in the bands and precipitates