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Polyphenol oxidase activity staining in polyacrylamide electrophoresis gels

✍ Scribed by Antonio Rescigno; Francesca Sollai; Andrea C Rinaldi; Giulia Soddu; Enrico Sanjust


Book ID
117331436
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
364 KB
Volume
34
Category
Article
ISSN
0165-022X

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A method for detecting the activity of ribonuclease inhibitors (RIs) after nondenaturing polyacrylamide gel electrophoresis and isoelectric focusing was developed. Both types of electrophoresis were performed using vertical slab polyacrylamide gels in the presence of dithiothreitol and in a sealed s