Stable preservation of hemoglobin vesicles as a blood substitute

✍ Scribed by Kazuhiro Akama; W.-L. Gong; Lin Wang; Satoru Tokuyama; Eishun Tsuchida

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 131 KB
• Volume: 10
• Category: Article
• ISSN: 1042-7147
• DOI: 10.1002/(sici)1099-1581(199905)10:5<293::aid-pat878>3.0.co;2-n

✦ Synopsis

Long-term preservation of hemoglobin (Hb) vesicles, the so-called artificial red cell (ARC), in dry powder was studied. Carbonylhemoglobin (COHb) was encapsulated in the vesicle of 1,2-bis(2,4-octadecadienoyl)-sn-glycero-3-phosphocholine (DODPC) and the polymerizable membrane components were polymerized by g-ray irradiation. The obtained ARC suspension was then freeze-dried in the presence of sucrose. The factors influencing the shelf-life of the freeze-dried ARC, such as sucrose concentration, moisture and storage temperature, were elucidated. After storage in the dry state for more than one year, the oxygen-carrying capacity was recovered by rehydration of the freeze-dried ARC with distilled water and substitution of the carbon monoxide ligand with oxygen.